Genome sequence Bacillus velezensis LZN01 is a M.Tech project topic for Biotechnology & Biomedical Engineering. It gives students a clear starting point for research, implementation planning, and documentation.
Genome sequence Bacillus velezensis LZN01 Project Details
| Abstract |
This project builds a complete computational workflow for assembling and annotating the genome of *Bacillus velezensis* strain LZN01, a strong biocontrol agent against the fungus *Fusarium oxysporum* f. sp. niveum. The workflow uses a hybrid sequencing strategy: shortβread data from an Illumina HiSeq platform combined with longβread data from PacBio. With the Unicycler assembly pipeline, these reads are merged to produce a finished, circular genome. After assembly, the genome is annotated to locate: * coding DNA sequences (CDSs) * ribosomal RNAs (rRNAs) * transfer RNAs (tRNAs) A phylogenomic analysis then checks that the strain is correctly classified as *B. velezensis*. The antiSMASH tool is run to find secondaryβmetabolite biosynthetic gene
clusters (BGCs). The analysis highlights clusters that make known antimicrobial compounds, including: * surfactin * fengycin * bacillibactin * bacilysin To explore how the strain might produce the compound myriocin, metabolicβpathway reconstruction is performed. This step points to several candidate genes that could be involved, such as **epsN**, **capD**, **dagK**, **glk**, and **manA**. Overall, the computational model provides a detailed reference for studying plantβmicrobe interactions, biofilm formation, and plantβgrowthβpromoting traits at the genome level. It offers clear, stepβbyβstep guidance for M.Tech students working in agricultural biotechnology and bioinformatics.
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| Reference Paper |
Genome sequence of Bacillus velezensis LZN01 inhibiting Fusarium oxysporum f. sp. niveum and producing myriocin |
| Domain |
Biotechnology & Biomedical Engineering |
| Sub-Domain |
Computational Biology / Bioinformatics / Genome Assembly |
| PDF Download |
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| Get Help |
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